MD Anderson Cancer Center Research Assistant I -Molecular Biology in Houston, Texas
The University of Texas MD Anderson Cancer Center in Houston is one of the world's most respected centers focused on cancer patient care, research, education and prevention. It was named the nation's No. 1 hospital for cancer care in U.S. News & World Report's 2019 rankings.
Ideal candidates will have basic cellular and molecular biology skills, and/or RNA and HPV research experience
Study Data - Patient Eligibility/Informed Consents
Assists in the procurement of tissue/specimen and related data for basic translational and clinical research protocols. Tracks protocol related labs, responses and research tests. Under the supervision of the medical staff, performs protocol specific tasks including screening, ordering tests and study documentation of patient collected samples/specimen.
Collects Research Samples
Collects or obtains research samples including blood, urine, saliva and biopsy, and or tissue biopsies using routine phlebotomy procedures or other techniques. Processes research samples according to the standard operating procedure (SOP). Remains knowledgeable of all policies and procedures that are essential to the collection, processing, transportation, storage, and/or shipping of research samples. Packages biologic samples and coordinates shipment with courier services.
Assists with the request and transfer of samples to and from outside centers. Maintains a file system to document the progress and status of each request. Locates, files, and/or retrieves the required documents for tissue requests in the regulatory file.
Develops, revises and/or designs documents for tissue acquisition and data storage/retrieval. Assists with determination of noncoding RNAs (miRNAs and long noncoding RNAs, and HPV from human samples (serum and tissues). Assists with maintenance of cultured cells. Assists with extraction and preparation of DNA/RNA.
Responsible for Conducting Daily Animal Work
Responsible for Conducting Daily Animal Work Responsible for conducting daily animal work using mouse and egg tumor models including
a. Maintains mouse colony
b. Performs mouse tagging and genotyping
c. Injects tumor or normal cells into tongues, body flanks and tail veins.
d. Implants patient derived tumors (PDX) into the flank.
e. Administer agents via IP, oral gavage and tail vein to study tumor growth inhibition with and without agent treatment.
f. Performs immunohistochemistry on frozen and paraffin tissue sections.
g. In charge of login in-vivo data into frozen and paraffin tissue database.
h. Performs imaging and monitors all animals
i. Performs necropsy and collects tumors and tissues from mice.
j. Performs treatment and survival mice studies.
k. Enters complete mouse information and experimental conditions in database
l. Acts as the contact person between the lab and the Vet Department.
m. Trains and assists all personnel with mice experiments, policies and procedures.
n. Carries out duties required for maintenance and preparation of embryonated eggs, and implantation of tumors
o. Performs experiments on egg tumor models, including various treatments, imaging, and other real-time assays
p. Harvests and prepares specimens from egg tumors
q. Performs various downstream molecular and immunologic assays resulting from mouse and egg procedures, as needed.
Cell culture and in vitro experiments
a. Maintains and stores established and primary cells under the appropriate sterile, storage and safety conditions including testing and treating for mycoplasma.
b. Maintains a safe and clean work area for the culturing of the various cells and biological materials.
c. Stocks and maintains an up-to-date record of cultured cells
d. Performs analysis of protein and RNA expression
e. Applies molecular biology techniques to the generation of DNA constructs
f. Requires experience in tissue culture, DNA & RNA preparation and cloning, PCR, protein synthesis and electrophoresis, MTT and clonogenic assays. Responsible for communicating experimental design and results
Safety and Regulatory Compliance
Assists, and or writes and submits all hazardous and non-hazardous agents to the Institution Biosafety Committee (IBC) in addition to all animal protocols to the Animal Care Committee (IACUC) as requested by the Principal Investigator.
a. Assemble samples, reagents, dry or wet ice, data and packaging materials; draft a cover letter, accurately and legibly fill out shipping labels Keep accurate legible logs of outgoing and incoming packages. Ship all Hazardous and Infectious Materials in accordance with USA and Institutional policy.
Bachelor's degree, major course work in one of the basic sciences.
Basic cellular and molecular biology skills
RNA and HPV research experience
It is the policy of The University of Texas MD Anderson Cancer Center to provide equal employment opportunity without regard to race, color, religion, age, national origin, sex, gender, sexual orientation, gender identity/expression, disability, protected veteran status, genetic information, or any other basis protected by institutional policy or by federal, state or local laws unless such distinction is required by law. http://www.mdanderson.org/about-us/legal-and-policy/legal-statements/eeo-affirmative-action.html
Requisition ID: 139507
Employment Status: Full-Time
Employee Status: Regular
FLSA: non-exempt, eligible for overtime, and is subject to the provisions of the Fair Labor Standards Act (FLSA)
Work Week: Days
Fund Type: Soft
Pivotal Position: No
Minimum Salary: US Dollar (USD) 30,400
Midpoint Salary: US Dollar (USD) 38,000
Maximum Salary : US Dollar (USD) 45,600
Science Jobs: Yes