MD Anderson Cancer Center Research Assistant I in Houston, Texas
JOB SPECIFIC COMPETENCIES
-Functions, under supervision, in the design and initiation of research experiments. These experiments include, but are not limited to, DNA/RNA isolation from human and murine plasma, tumor tissues and cell lines, flow cytometry, PCR, RT-PCR, Western blotting, immunohistochemistry, protein purification, and other approaches.
Laboratory Support, Record Keeping and Data Collection
-Assists with basic laboratory techniques and in preparation of media solutions, buffers and reagents.
-Reads literature and confers with superiors regarding new procedures and modifies them according to the needs of the project.
-Keep ample stock of supplies on hand
-Order reagents and materials when needed.
-Know the procedure for obtaining repair or maintenance for lab equipment
-Prepare stock solutions
-Perform laboratory maintenance, such as cleaning
To maintain good, detailed experimental records, evaluate resulting research data, and present findings.
-Maintain an up-to-date, organized, and legible laboratory journal that records all experiments in detail and ensure that subordinates do the same.
-Utilize image analysis system and/or microscope to analyze data and examine results.
-Compile, write, and submit project results to supervisor.
Work closely with other staff members
-Maintain good working relationship with other team members
-Explain to staff members the scientific protocols currently being employed in the laboratory.
-Receive verbal and/or written instructions from principal investigator on standards and specialized lab safety procedures.
-Attend annual safety courses required by MDA and other agencies. Handle, store, and dispose of radioactive isotopes, chemically hazardous materials, and bio hazardous materials in procedure specified to meet MDA safety guidelines.
-Follow manufacturer's guidelines regarding the handling and maintenance of all lab equipment. Have working knowledge of equipment operation manuals and procedure manuals whenever necessary.
-Monitor workflow as it pertains to the need for supplies, equipment maintenance, and preparing stock solutions
Handle all aspects of the mouse experiments
-Monitor the animals on a regular basis
-Conduct experiments by injecting cancer cells into animals, monitoring for toxicity and performing autopsies.
-Treat animals with therapeutic agents and monitor for toxicity and efficacy of treatments.
-Perform staining and flow cytometry assays of immune cells from tissues harvested from autopsies
-Perform IHC, protein or RNA analyses of tissue samples taken from animals
-Maintain and establish culture of human and murine cells, which require rigorous sterile techniques. Observe and identify cell changes under the microscope, determine cell counts and cell viability.
-Prepare sterile media and solutions.
-Purify cells by various methods and monitor cells for proliferation and activation by standard laboratory methods.
-Monitor supplies, order and store tissue culture supplies to ensure efficient functioning.
-Prepare cells for cryogenic storage and keep them organized and updated on the laboratory inventoried cell stock list.
-The above requires using the microscope for prolonged periods, working for prolonged periods with hands extended under a flow hood and lifting cases (<50lbs>
Other duties as assigned
Education Required: Bachelor's degree, major course work in one of the basic sciences.
Experience Required: No experience.
It is the policy of The University of Texas MD Anderson Cancer Center to provide equal employment opportunity without regard to race, color, religion, age, national origin, sex, gender, sexual orientation, gender identity/expression, disability, protected veteran status, genetic information, or any other basis protected by institutional policy or by federal, state or local laws unless such distinction is required by law. http://www.mdanderson.org/about-us/legal-and-policy/legal-statements/eeo-affirmative-action.html
Requisition ID: 150075
Employment Status: Full-Time
Employee Status: Regular
FLSA: non-exempt, eligible for overtime, and is subject to the provisions of the Fair Labor Standards Act (FLSA)
Work Week: Days
Fund Type: Soft
Work Location: Onsite
Pivotal Position: Yes
Minimum Salary: US Dollar (USD) 31,200
Midpoint Salary: US Dollar (USD) 39,000
Maximum Salary : US Dollar (USD) 46,800
Science Jobs: Yes