MD Anderson Cancer Center Research Assistant II - Leukemia in Houston, Texas
The department of Leukemia is dedicated to quality patient care, innovative research, and developing more effective treatments for all types of leukemia. Clinical research conducted by our faculty and staff consistently translates into new leukemia therapies and advances overall knowledge of the disease.
The Research Assistant II provides technical and coordination skills in carrying out various research projects.
The salary range is: min-$34,400, mid-$43,000 and max-$51,600.
Processing Tissue Cultures
· Receives human tissue and fluid specimen in inpatient services and outpatient clinic.
· Separates serum, plasma, peripheral blood leukocytes and tumor cells.
· Maintains and establishes tissue culture cell lines which require good sterile techniques and the ability to observe and identify cell changes under the microscope, determine cell counts on the hemocytometer or coulter counter, performs colony counts or determine cell viability.
· Determines cell counts on the hemocytometer or coulter counter, performs colony counts or determines cell viability.
· Purifies leukocytes and tumor cell populations using ficoll density gradients and immunoaffinity techniques.
· Uses aseptic techniques and biosafety measures.
· Prepares sterile media and solutions.
· Monitors and shelves tissue culture supplies to ensure efficient functioning.
· Prepares cells for cryogenic storage and reestablishment by the use of small vials, dry ice and liquid nitrogen.
· Meets time and attendance expectations and responds to requests in a timely manner, communicating expectations for procedures, service arrival, or project deliverables to patients and coworkers.
· Must possess good manual dexterity.
· The above requires sitting and using the microscope for long periods of time, working for long periods with hands extended under a flow hood and lifting cases of supplies or up to 10 liters on a cart, as well as moving around the lab to work different equipment.
Laboratory Equipment and Maintenance
· Performs routine maintenance of ultra-low freezers, liquid nitrogen, C02 and water levels.
· Should be able to maintain equipment such as high-speed centrifuges, PH meters and microfuges.
· Manually operates and maintains the lab equipment.
· Visually reads equipment operation manuals whenever necessary.
· Must be able to lift and carry items up to 20-25 pounds and carry or move from one area of the lab to another by using a roller cart.
· Models safe, ethical behavior, mitigating risk to the institution through sound business practices and adherence to MD Anderson's Standards of Conduct and institutional policies and procedures. Must be able to comply with Universal Precautions and OSHA standards when handling toxic, hazardous, flammable, radioactive or potentially infectious or carcinogenic materials. This may require wearing common protective garments or equipment such as gloves, eye protection, face mask, gown or lab coat, etc.
· Additional some procedures may require the use of a fume hood or biological safety cabinet.
· Cleans laboratory benches as required.
· Shares in general maintenance of laboratory including monitoring radioisotopes and aliquot reagents.
· Isolates, purifies and analyzes RNA, DNA and protein including hybridization, gel electrophoresis autoradiography, ECL staining, performing PCR reactions, quantitative RT-PCR assay, DNA sequencing, Western blot analysis, CRISPR/CAS9 gene editing, molecular cloning and assays for signal transducing enzymes.
· Purification and conjugation of immunoglobulins.
· Should have basic knowledge of solution and buffer preparation in which ionic concentration and pH is critical.
· Should have basic training in use and safety of radioisotopes.
· Should have basic training to maintain mouse colony. Collect and store mouse tissue as needed.
Require: Bachelor's degree with major course works in one of the basic sciences.
Required: Two years experience in scientific or experimental research work. No experience required with preferred degree.
It is the policy of The University of Texas MD Anderson Cancer Center to provide equal employment opportunity without regard to race, color, religion, age, national origin, sex, gender, sexual orientation, gender identity/expression, disability, protected veteran status, genetic information, or any other basis protected by institutional policy or by federal, state or local laws unless such distinction is required by law. http://www.mdanderson.org/about-us/legal-and-policy/legal-statements/eeo-affirmative-action.html